Biotransformation of some steroids by Mucor hiemalis MRC 70325

Abstract

In this work, incubations of testosterone, dehydroepiandrosterone and pregnenolone with Mucor hiemalis MRC 70325 have been reported. Incubation of testosterone afforded androst-4-en-3,17-dione (3%), 14 alpha-hydroxyandrost-4- en-3,17-dione (9%), 6 beta-hydroxyandrost-4-en-3,17-dione (2%) and 14 alpha, 17 beta-dihydroxyandrost-4-en-3-one (62%). Incubation of dehydroepiandrosterone afforded 3 beta, 17 beta-dihydroxyandrost-5-ene (6%) and 3 beta, 7 alpha-dihydroxyandrost-5-en- 17-one (72%). Incubation of pregnenolone afforded 3 beta, 14 alpha-dihydroxypregn-5-en-7,20-dione (3%), 3 beta, 7 alpha-dihydroxy-pregn-5-en-20-one (64%) and 3 beta, 7 alpha, 11 alpha-trihydroxypregn-5-en-20-one (11%). 3 beta, 14 alpha-Dihydroxypregn-5-en-7,20-dione was identified as a new metabolite.