dc.contributor.authors |
Kuru, Ali; Yildirim, Kudret |
|
dc.date.accessioned |
2022-12-20T13:25:42Z |
|
dc.date.available |
2022-12-20T13:25:42Z |
|
dc.date.issued |
2022 |
|
dc.identifier.issn |
1024-2422 |
|
dc.identifier.uri |
http://dx.doi.org/10.1080/10242422.2022.2150967 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12619/99418 |
|
dc.description |
Bu yayının lisans anlaşması koşulları tam metin açık erişimine izin vermemektedir. |
|
dc.description.abstract |
In this work, biotransformations of pregnenolone 1 by Ulocladium chartarum MRC 72584, Cladosporium sphaerospermum MRC 70266 and Cladosporium cladosporioides MRC 70282 have been reported. U. chartarum MRC 72584 only hydroxylated 1 at C-7 beta and hydroxylated 1 at either C-7 beta or C-14 alpha, accompanied with a concurrent epoxidation from the beta-face. The same fungus hydroxylated a small amount of 1 at both C-7 and C-14 alpha, accompanied with a subsequent oxidation at C-7. 3 beta,14 alpha-Dihydroxy-5 beta,6 beta-epoxypregnan-20-one 4 and 3 beta,7 beta-dihydroxy-5 beta,6 beta-epoxypregnan-20-one 5 obtained from the incubation of 1 with this fungus were determined as new metabolites. C. sphaerospermum MRC 70266 converted most of 1 into a 3-keto-4-ene steroid and independently hydroxylated it at C-6 alpha, C-6 beta and C-7 beta. This fungus also hydroxylated 1 at C-7 and C-11 alpha and then oxidized it at C-7. C. cladosporioides MRC 70282 converted almost half of 1 into a 3-keto-4-ene steroid. The same fungus reduced some of this 3-keto-4-ene steroid by a 5 alpha-reductase activity while it hydroxylated the rest at C-6 alpha and C-6 beta. |
|
dc.language |
English |
|
dc.language.iso |
eng |
|
dc.relation.isversionof |
10.1080/10242422.2022.2150967 |
|
dc.subject |
Biochemistry & Molecular Biology |
|
dc.subject |
Biotechnology & Applied Microbiology |
|
dc.subject |
Ulocladium chartarum |
|
dc.subject |
Cladosporium sphaerospermum |
|
dc.subject |
Cladosporium cladosporioides |
|
dc.subject |
pregnenolone |
|
dc.subject |
biotransformation |
|
dc.title |
Microbial conversion of pregnenolone by some filamentous fungi |
|
dc.type |
Early Access |
|
dc.relation.journal |
BIOCATALYSIS AND BIOTRANSFORMATION |
|
dc.identifier.doi |
10.1080/10242422.2022.2150967 |
|
dc.identifier.eissn |
1029-2446 |
|
dc.contributor.author |
Kuru, Ali |
|
dc.contributor.author |
Yildirim, Kudret |
|
dc.relation.publicationcategory |
Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı |
|