dc.date.accessioned |
2021-06-08T09:12:06Z |
|
dc.date.available |
2021-06-08T09:12:06Z |
|
dc.date.issued |
2020 |
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dc.identifier.issn |
0892-3973 |
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dc.identifier.uri |
https://hdl.handle.net/20.500.12619/96198 |
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dc.description |
This work was supported by the Scientific and Technological Research Council of Turkey, [TUBITAK] under Grant [number 1002-116S102]. |
|
dc.description |
Bu yayının lisans anlaşması koşulları tam metin açık erişimine izin vermemektedir. |
|
dc.description.abstract |
Background: Toll-like receptors (TLRs) are often expressed in natural immune cells as well as in tumor cells. TLR4 exhibits both tumor promoting and tumor-suppressing roles and higher TLR9 expression is an important marker of poor prognosis in prostate cancer (PCa). Nobiletin (NOB) is an O-methylated flavonoid and NOB has been proven to have anti-cancer effect in PCa cells. However, there is no study in the literature investigating the potential anti-inflammatory effects of NOB on the TLR signaling pathways in cancer. Therefore, we aimed to explore the potential anti-inflammatory effects of NOB on the TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in different types of PCa cell lines, for the first time. Material and methods: In the current study, the cytotoxic effect of NOB PC-3 (hormone-independent and metastatic) and LNCaP cells (hormone-dependent) was evaluated by WST-1 assay. Furthermore, the inhibitory effects of NOB on TLR4/TRIF/IRF3 and TLR9/IRF7signaling pathway were determined by RT-PCR, western blotting and ELISA analysis. Results: NOB demonstrated an inhibitory effect on PCa cell growth and LNCaP cells were more sensitive to NOB than PC-3 cells due to androjen receptor status. Furthermore, NOB alone could suppress TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways through the downregulation of their associated pathways (mRNA and related protein levels) and the release of IFN-alpha and IFN-beta compared to LPS or CpG-ODN stimulated PCa cells. Conclusions: NOB potentially inhibited TLR4 and TL9-dependent signaling pathway in PCa cells. However, the efficacy of NOB was different in PCa cells due to the hormone status and aggressive features. |
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dc.description.sponsorship |
Scientific and Technological Research Council of Turkey, [TUBITAK]Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [1002-116S102] |
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dc.language |
English |
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dc.language.iso |
eng |
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dc.publisher |
TAYLOR & FRANCIS LTD |
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dc.relation.isversionof |
10.1080/08923973.2020.1725040 |
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dc.rights |
info:eu-repo/semantics/closedAccess |
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dc.subject |
TOLL-LIKE RECEPTOR |
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dc.subject |
SOLUBLE INTERLEUKIN-2-RECEPTOR |
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dc.subject |
CITRUS NOBILETIN |
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dc.subject |
ANGIOGENESIS |
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dc.subject |
INFLAMMATION |
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dc.subject |
EXPRESSION |
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dc.subject |
SURVIVAL |
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dc.subject |
INVASION |
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dc.title |
Anti-inflammatory effects of nobiletin on TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in prostate cancer cells |
|
dc.type |
Article |
|
dc.contributor.authorID |
guney eskiler, gamze/0000-0002-2088-9914 |
|
dc.contributor.authorID |
Deveci Ozkan, Asuman/0000-0002-3248-4279 |
|
dc.contributor.authorID |
ONEN, HACER ILKE/0000-0002-7220-0066 |
|
dc.contributor.authorID |
Kalayci Yigin, Aysel/0000-0001-8549-5564 |
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dc.identifier.volume |
42 |
|
dc.identifier.startpage |
93 |
|
dc.identifier.endpage |
100 |
|
dc.relation.journal |
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY |
|
dc.identifier.issue |
2 |
|
dc.identifier.doi |
10.1080/08923973.2020.1725040 |
|
dc.identifier.eissn |
1532-2513 |
|
dc.contributor.author |
Deveci Ozkan, Asuman |
|
dc.contributor.author |
Kaleli, Suleyman |
|
dc.contributor.author |
Onen, Hacer Ilke |
|
dc.contributor.author |
Sarihan, Mehmet |
|
dc.contributor.author |
Guney Eskiler, Gamze |
|
dc.contributor.author |
Kalayci Yigin, Aysel |
|
dc.contributor.author |
Akdogan, Mehmet |
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dc.relation.publicationcategory |
Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı |
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dc.identifier.pmıd |
32048561 |
|