dc.date.accessioned |
2021-06-04T08:06:12Z |
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dc.date.available |
2021-06-04T08:06:12Z |
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dc.date.issued |
2021 |
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dc.identifier.issn |
0308-8146 |
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dc.identifier.uri |
https://hdl.handle.net/20.500.12619/95695 |
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dc.description |
M.O. thanks the Turkish Academy of Sciences (TUBA) for partial support. |
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dc.description |
Bu yayının lisans anlaşması koşulları tam metin açık erişimine izin vermemektedir. |
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dc.description.abstract |
Metal-organic structures (MOFs) have been designed for a wide range of applications due to their high porosity, large surface area, and flexibility. For the first time in this work, the successful immobilization of alpha-amylase is confirmed by the use of ZIF-8 as easy and good support. The morphology, functional groups, and chemical composition of the support and immobilized a-amylase were tested using different methods such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermal gravimetric analysis (TGA). The enzymatic activities of the immobilized olibanum-bovine serum albumin@zeolitic imidazolate frameworks nanocomposite (OLB/BSA@ZIF-8)-alpha-amylase were compared with the free one. The pH and thermal stability of the OLB/BSA@ZIF-8-alpha-amylase were significantly enhanced compared to the free enzyme. The OLB/ BSA@ZIF-8-alpha-amylase displayed excellent long-term storage stability, which could protect more than 90% of the initial activity for 8 weeks. Besides, the OLB/BSA@ZIF-8-alpha-amylase had high reusability, which showed a high degree of activity (more than 81%) after 20 cycles. This is the first study that uses OLB/BSA@ZIF-8 nanocomposite as immobilizing support for the immobilization of alpha-amylase. Improved catalytic efficiency (V-max/K-m) values, reusability, and storage stability of immobilized a-amylase can make it suitable in industrial and biotechnological applications. |
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dc.description.sponsorship |
Turkish Academy of Sciences (TUBA)Turkish Academy of Sciences |
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dc.language |
English |
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dc.language |
İngilizce |
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dc.language.iso |
eng |
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dc.publisher |
ELSEVIER SCI LTD |
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dc.rights |
info:eu-repo/semantics/closedAccess |
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dc.title |
( )Immobilization of alpha-amylase enzyme on a protein @metal-organic framework nanocomposite: A new strategy to develop the reusability and stability of the enzyme |
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dc.type |
Article |
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dc.identifier.volume |
349 |
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dc.relation.journal |
FOOD CHEMISTRY |
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dc.identifier.wos |
WOS:000621598600011 |
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dc.identifier.doi |
10.1016/j.foodchem.2021.129127 |
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dc.identifier.eissn |
1873-7072 |
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dc.contributor.author |
Atiroglu, Vesen |
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dc.contributor.author |
Atiroglu, Atheer |
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dc.contributor.author |
Ozacar, Mahmut |
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dc.relation.publicationcategory |
Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı |
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dc.identifier.pmıd |
33561794 |
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