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Utilization of Flow Cytometry in Immunologic and Allergic Diseases

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dc.contributor.authors Ozdemir, O; Karavalzoglu, C;
dc.date.accessioned 2020-02-27T08:26:04Z
dc.date.available 2020-02-27T08:26:04Z
dc.date.issued 2016
dc.identifier.citation Ozdemir, O; Karavalzoglu, C; (2016). Utilization of Flow Cytometry in Immunologic and Allergic Diseases. ASIM ALLERJI IMMUNOLOJI, 14, 128-117
dc.identifier.issn 1308-9234
dc.identifier.uri https://doi.org/10.21911/aai.5044
dc.identifier.uri https://hdl.handle.net/20.500.12619/65703
dc.description.abstract Flow cytometry is a device that analyzes the physical and/or chemical characteristics of cells or particles as they pass through a fluid system one by one. Laser beam diffracted and florescent light emitted by each cell or particle while passing through the fluid system are gathered by optic filters and mirrors, separated into varying wavelengths, and transformed into analogue signals. The signals are digitalized and transferred to a screen as histograms. The histograms are based on the principle of demonstrating the frequency distributions of the parameters measured visually. While the data obtained can be demonstrated in different graphics, the cells are separated according to cell size and granular structure. Analysis of cell groups through fluorescently labeled antibodies enabling analysis of more than one surface antigen at the same time can also be performed by flow cytometry. Today, immunologic diseases are one of the most common fields where flow cytometry is used. Monoclonal antibodies used to detect lymphocyte subgroups in flow cytometry are crucial in the diagnosis of disease groups and types. Examples of some types of immunodeficiency diagnosed by flow cytometry are given in this study. Immunologic diseases such as severe combined immunodeficiency, Bruton's disease, chronic granulomatous disease, common variable immunodeficiency and bare lymphocyte syndrome can be assessed with flow cytometry. Its use in allergic diseases is based on measuring basophil activation in flow cytometry. When the basophil contacts with antigen, the cell activates and expresses CD63 on the surface. Besides, there are clinical studies showing that the basophil activation test can be used to monitor the effectiveness of allergen immunotherapy. In near future, flow cytometry will be continued to be used more intensely in the diagnosis and follow-up of allergic and immunologic diseases with new markers and more developed technology.
dc.language Turkish
dc.subject Allergy
dc.title Utilization of Flow Cytometry in Immunologic and Allergic Diseases
dc.type Review
dc.identifier.volume 14
dc.identifier.startpage 117
dc.identifier.endpage 128
dc.contributor.department Sakarya Üniversitesi/Tıp Fakültesi/Dahili Tıp Bilimleri Bölümü
dc.contributor.saüauthor Özdemir, Öner
dc.relation.journal ASIM ALLERJI IMMUNOLOJI
dc.identifier.wos WOS:000404965600003
dc.identifier.doi 10.21911/aai.5044
dc.contributor.author Özdemir, Öner
dc.contributor.author Cagla Karavalzoglu

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