Açık Akademik Arşiv Sistemi

Inhibition of TLR4/TRIF/IRF3 Signaling Pathway by Curcumin in Breast Cancer Cells

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dc.contributor.authors Eskiler, GG; Ozkan, AD; Kaleli, S; Bilir, C;
dc.date.accessioned 2020-02-27T07:22:33Z
dc.date.available 2020-02-27T07:22:33Z
dc.date.issued 2019
dc.identifier.citation Eskiler, GG; Ozkan, AD; Kaleli, S; Bilir, C; (2019). Inhibition of TLR4/TRIF/IRF3 Signaling Pathway by Curcumin in Breast Cancer Cells. JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES, 22, 291-281
dc.identifier.issn 1482-1826
dc.identifier.uri https://hdl.handle.net/20.500.12619/65614
dc.identifier.uri https://doi.org/000474410600001
dc.description.abstract Purpose: Toll-like receptor 4 (TLR4) is over-expressed in breast tumors and thus contributing to the tumor progression and metastasis. Natural products have drawn attention in cancer immunotherapy due to their various biological activities. Curcumin is well investigated in different types of cancer. However, the mechanisms underlying its anti-inflammatory actions have not been extensively elucidated. For this purpose, we explored the inhibitory effects of curcumin on lipopolysaccharide (LPS)-induced TLR4 dependent TRIF signaling pathway in two subtypes of breast cancer cell lines (MCF-7 and MDA-MB-231) in this study. Methods: In this context, the cytotoxicity of curcumin and LPS alone and the combination of curcumin with LPS on these cells was evaluated by WST-1 assay. The expression level of TLR4 and the release of type I interferon (IFN) levels were determined after treatment with curcumin and/or LPS by RT-PCR and ELISA analysis, respectively. Furthermore, the subcellular localization of TLR4 and interferon regulatory factor 3 (IRF3) were detected by immunofluorescence analysis. Results: Curcumin treatment suppressed breast cancer cells viabilities and the activation of TLR4-mediated TRIF signaling pathway by the downregulation of TLR4 and IRF3 expression levels and the inhibition of type I IFN (IFN-alpha/beta) levels induced by LPS. However, curcumin was more efficient in MDA-MB-231 cells than MCF-7 cells owing to its greater inhibitory efficacy in the LPS-enhanced TLR4 signaling pathway. Furthermore, IFN-alpha/beta levels induced by TLR4 and IRF3 were decreased in these cells following curcumin treatment. Conclusions: Consequently, these results demonstrated that the activation of LPS stimulated TLR4/TRIF/IRF3 signaling pathway was mediated by curcumin in breast cancer cells, in vitro. However, more studies are necessary to examine the curcumin's anti-inflammatory activities on TLR4/MyD88/NF-kappa B as well as other signaling pathways downstream of TLRs in breast cancer.
dc.language English
dc.publisher CANADIAN SOC PHARMACEUTICAL SCIENCES
dc.subject Pharmacology & Pharmacy
dc.title Inhibition of TLR4/TRIF/IRF3 Signaling Pathway by Curcumin in Breast Cancer Cells
dc.type Article
dc.identifier.volume 22
dc.identifier.startpage 281
dc.identifier.endpage 291
dc.contributor.department Sakarya Üniversitesi/Tıp Fakültesi/Temel Tıp Bilimleri Bölümü
dc.contributor.saüauthor Güney Eskiler, Gamze
dc.contributor.saüauthor Deveci Özkan, Asuman
dc.contributor.saüauthor Kaleli, Süleyman
dc.contributor.saüauthor Bilir, Cemil
dc.relation.journal JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES
dc.identifier.wos WOS:000474410600001
dc.contributor.author Güney Eskiler, Gamze
dc.contributor.author Deveci Özkan, Asuman
dc.contributor.author Kaleli, Süleyman
dc.contributor.author Bilir, Cemil


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