Comparison of the effects of topical corneal inhibitory agents on TTL and PON1 in rats

Abstract

The aim of this study was to compare the effects of topical corneal inhibitory agents on total thiol (TTL) and paraoxonase 1 (PON1) levels in rats with experimentally acquired keratoconjunctivitis. Thirty-five rats were divided into five groups. Twenty-four hours prior to the experiment, keratoconjunctivitis was established in the right eye of the rats using sodium hydroxide. The treatments of the five groups were as follows: group I: (control) isotonic saline (0.9%), group II: topical 0.05% cyclosporine A, group III: topical 1% diluted propolis, group IV: topical 3% diluted propolis, and group V: 0.1% dexamethasone. At the end of the 10th day, one rat in each group, except the cyclosporine group (group II), had died. The treatment was applied to all groups three times a day for 10 days. Subsequently, blood samples were obtained and used for determining the levels of TTL and PON1 (Architect C16000). All statistical analyses were performed using IBM SPSS for Windows Version 20.0 software. Descriptive statistics were calculated from the values obtained from this study and shown as arithmetic mean and standard deviation. Kruskal Wallis variance analysis was conducted. p values found to be under 0.05 were accepted as statistically significant. The study was performed after the approval [By The Animal Research Ethics Committee, Bolu Abant Izzet Baysal University, Number: 13.30.2.ABU.0.05.05-050.01.04-1, January.8.2016]. The TTL results were as follows (mu mol/L): group I: 253.24, group II: 238.70, group III: 281.39, group IV: 284.80 and group V: 260.65. No marked differences were observed between the control group and the other groups (P>0.05). The PON1 results were as follows (U/L): group I: 521.49, group II: 472.30, group III: 362.37, group IV: 327.48 and group V: 440.31. No marked difference was observed between the control group and the other groups (P > 0.05). However, there was a marked difference in the PON1 results between the 1% and 3% propolis groups. CNV inhibitor agents' effects on TTL and PON1 values were similar.