Açık Akademik Arşiv Sistemi

Tumor-specific mutation and downregulation of ING5 detected in oral squamous cell carcinoma

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dc.contributor.authors Cengiz, B; Gunduz, E; Gunduz, M; Beder, LB; Tamamura, R; Bagci, C; Yamanaka, N; Shimizu, K; Nagatsuka, H;
dc.date.accessioned 2020-02-27T07:16:54Z
dc.date.available 2020-02-27T07:16:54Z
dc.date.issued 2010
dc.identifier.citation Cengiz, B; Gunduz, E; Gunduz, M; Beder, LB; Tamamura, R; Bagci, C; Yamanaka, N; Shimizu, K; Nagatsuka, H; (2010). Tumor-specific mutation and downregulation of ING5 detected in oral squamous cell carcinoma. INTERNATIONAL JOURNAL OF CANCER, 127, 2094-2088
dc.identifier.issn 0020-7136
dc.identifier.uri https://doi.org/10.1002/ijc.25224
dc.identifier.uri https://hdl.handle.net/20.500.12619/65193
dc.description.abstract Our previous study showed high frequency of allelic loss at chromosome 2q37 region in oral cancer. This location contains several candidate tumor suppressor genes such as PPP1R7, ILKAP, DTYMK and ING5. We previously showed 3 members of inhibitor of growth (ING) family, ING1, ING3 and ING4 as tumor suppressor gene in head and neck cancer. As ING5 shows high homology with other members of ING genes including highly conserved carboxy-terminal plant homeodomain and nuclear localization signal, we first picked up ING5 and examined it as a possible tumor suppressor in oral cancer. For this aim, mutation and mRNA expression status of ING5 in paired normal and oral squamous cell carcinoma samples were examined by reverse transcription polymerase chain reaction (RT-PCR) and sequencing. Three missense mutations located within leucine zipper like (LZL) finger and novel conserved region (NCR) domains in ING5 protein were detected, probably abrogating its normal function. We also found 5 different alternative splicing variants of ING5. Then, we examined mRNA level of ING5 by quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) analysis, which demonstrated decreased expression of ING5 mRNA in 61% of the primary tumors as compared to the matched normal samples. In conclusion, tumor-specific mutation and downregulation of ING5 mRNA suggested it as a tumor suppressor gene in oral squamous cell carcinoma.
dc.language English
dc.publisher WILEY
dc.title Tumor-specific mutation and downregulation of ING5 detected in oral squamous cell carcinoma
dc.type Article
dc.identifier.volume 127
dc.identifier.startpage 2088
dc.identifier.endpage 2094
dc.contributor.department Sakarya Üniversitesi/Mühendislik Fakültesi/Makine Mühendisliği Bölümü
dc.contributor.saüauthor Gündüz, Mehmet
dc.contributor.saüauthor Bağcı, Cahit
dc.relation.journal INTERNATIONAL JOURNAL OF CANCER
dc.identifier.wos WOS:000282539600010
dc.identifier.doi 10.1002/ijc.25224
dc.contributor.author Beyhan Cengiz
dc.contributor.author Esra Gunduz
dc.contributor.author Gündüz, Mehmet
dc.contributor.author Levent Bekir Beder
dc.contributor.author Ryo Tamamura
dc.contributor.author Bağcı, Cahit
dc.contributor.author Noboru Yamanaka
dc.contributor.author Kenji Shimizu
dc.contributor.author Hitoshi Nagatsuka


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