Immobilization of Polyphenol Oxidase from Princess Tree Leaf and its applications in Dye Decolorization

Abstract

In this work, Polyphenol oxidase (PPO) enzyme was extracted and partially purified from princess tree leaves (Paulownia tomentosa). Then, it was immobilized on calcium alginate beads. The free and immobilized PPO enzymes were applied for the decolorization of metal complex and acid dyes. The metal complex and acid dyes in water were mixed with free and immobilized princess tree PPO for one hour. The percent of decolorization was determined by using untreated dye mixture. Immobilized PPO from princess tree leaf was considerably more active in decolorzation of the dyes than the free PPO. Our results suggested that the immobilized princess tree PPO could be useful for removing artificial dyes from industrial wastes.