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A comparison of Polyphenoloxidases from different plants

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dc.contributor.authors Arabaci, G; Duzcan, B;
dc.date.accessioned 2020-02-24T14:14:20Z
dc.date.available 2020-02-24T14:14:20Z
dc.date.issued 2009
dc.identifier.citation Arabaci, G; Duzcan, B; (2009). A comparison of Polyphenoloxidases from different plants. FEBS JOURNAL, 276, 128-128
dc.identifier.issn 1742-464X
dc.identifier.uri https://hdl.handle.net/20.500.12619/44724
dc.identifier.uri https://doi.org/10.1080/10942910903453363
dc.description.abstract Artichoke polyphenol oxidases (PPOs) were obtained by (NH4)2SO4 precipitation using ascorbic acid, polyvinylpyrrolidone, and Triton X-100. The PPO content of artichoke head (AHPPO) was 8820 units (mg protein)−1 as compared with 3370 units (mg protein)−1 in artichoke leaves-and-stem (ALSPPO) by using catechol as a substrate. The substrates of both AHPPO and ALSPPO are o-diphenols, such as catechol, pyrogallol, and L-DOPA. Optimum pH and temperature of both PPOs were determined. AHPPO had higher thermal stability than ALSPPO. Also, Tm (the midpoint of thermal inactivation) and t1/2 (half-life) values were determined. Km and Vmax of both PPOs were observed to be similar. Twelve inhibitors were tested and their I50 values were determined. The most effective inhibitors were found to be potassium cyanide, ascorbic acid, L-cysteine, and thiourea. Sodiumdodecylsulfate, urea, and cupric sulfate caused an increase about 20–30% in the PPO activity.
dc.language English
dc.subject Biochemistry & Molecular Biology
dc.title A comparison of Polyphenoloxidases from different plants
dc.type Meeting Abstract
dc.identifier.volume 276
dc.identifier.startpage 128
dc.identifier.endpage 128
dc.contributor.department Sakarya Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü
dc.contributor.saüauthor Arabacı, Gülnur
dc.relation.journal FEBS JOURNAL
dc.identifier.wos WOS:000267069900353
dc.contributor.author Arabacı, Gülnur

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