Açık Akademik Arşiv Sistemi

Molecular characterization of sweet cherry genetic resources in Giresun, Turkey

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dc.contributor.authors Demir, T; Demirsoy, L; Demirsoy, H; Kacar, YA; Yilmaz, M; Macit, I
dc.date.accessioned 2020-03-06T08:32:36Z
dc.date.available 2020-03-06T08:32:36Z
dc.date.issued 2011
dc.identifier.citation Demir, T; Demirsoy, L; Demirsoy, H; Kacar, YA; Yilmaz, M; Macit, I (2011). Molecular characterization of sweet cherry genetic resources in Giresun, Turkey. FRUITS, 66, 62-53
dc.identifier.issn 0248-1294
dc.identifier.uri https://doi.org/10.1051/fruits/2010041
dc.identifier.uri https://hdl.handle.net/20.500.12619/67332
dc.description.abstract Introduction. Turkey potentially has a very rich source of sweet (Prunus avium) and sour (P. cerasus) cherries. P. avium is apparently native to some parts of Northern Turkey, where Giresun is located. Identification of the sweet cherry cultivars produced in Turkey will help in choosing appropriate cultivars and aid in the preservation of natural resources required for breeding studies. The most conventional method of cultivar identification is based on the assessment of morphological characteristics. However, this method is insufficient to distinguish closely related cultivars. The aims of our study were to determine the molecular profile of sweet cherry accessions grown in Giresun, Turkey, and to determine their genetic relationships. Materials and methods. In our study, we identified 44 sweet cherry accessions grown in Giresun by using genetic markers (SSR, Simple Sequence Repeat), and we determined the genetic relationships among the sweet cherry genotypes. For DNA isolation, we collected young leaves sampled on a single plant per accession, then amplification of microsatellite loci was performed. In total, ten SSR primer pairs, previously isolated from peach and sweet cherry, were used. Genetic similarity values were calculated. A cluster analysis was performed to generate a dendrogram. Results and discussion. Of the ten primers tested, six primer pairs did not result in suitable amplification products with the 44 accessions studied. The remaining four polymorphic SSR primer pairs produced 33 alleles with an average of 8.25 putative alleles per locus, ranging from 7 to 11. Depending on the accessions, similarity ratios ranged from 0.32 to 0.98, with a mean value of 0.64. In conclusion, the results obtained demonstrate a high level of polymorphism among sweet cherry genotypes from a single province in Turkey.
dc.language English
dc.publisher INT SOC HORTICULTURAL SCIENCE-ISHS
dc.title Molecular characterization of sweet cherry genetic resources in Giresun, Turkey
dc.type Article
dc.identifier.volume 66
dc.identifier.startpage 53
dc.identifier.endpage 62
dc.contributor.department Sakarya Üniversitesi/Mühendislik Fakültesi/Gıda Mühendisliği Bölümü
dc.contributor.saüauthor Demir, Taki
dc.relation.journal FRUITS
dc.identifier.wos WOS:000286505900007
dc.identifier.doi 10.1051/fruits/2010041
dc.identifier.eissn 1625-967X
dc.contributor.author Demir, Taki


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