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Effects of Immune Complexes on Holotranscobalamine Assay of Vitamin B-12 Deficiency in Myeloproliferative Disorders

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dc.contributor.authors Cekdemir, D; Cinemre, FBS; Aydemir, B; Dilaveroglu, N; Cekdemir, YE; Gunduz, M; Cinemre, H;
dc.date.accessioned 2020-02-27T08:28:15Z
dc.date.available 2020-02-27T08:28:15Z
dc.date.issued 2019
dc.identifier.citation Cekdemir, D; Cinemre, FBS; Aydemir, B; Dilaveroglu, N; Cekdemir, YE; Gunduz, M; Cinemre, H; (2019). Effects of Immune Complexes on Holotranscobalamine Assay of Vitamin B-12 Deficiency in Myeloproliferative Disorders. UHOD-ULUSLARARASI HEMATOLOJI-ONKOLOJI DERGISI, 29, 37-31
dc.identifier.issn 1306-133X
dc.identifier.uri https://doi.org/10.4999/uhod.193047
dc.identifier.uri https://hdl.handle.net/20.500.12619/66046
dc.description.abstract In myeloproliferative disorders (MPDs), vitamin B-12 levels are measured falsely elevated with conventional methods due to increased carrier protein synthesis. HoloTranscobalamine (HoloTC) assay is a first-choice method for detecting true vitamin B-12 deficiency in MPDs. Our aim was to determine effects of immune complexes on HoloTC assay. This is a cross-section study. Vitamin B-12 levels in 61 patients with myeloproliferative disorders were measured by both electrochemical immunoassay and HoloTC assay. The HoIoTC cutoff was greater than 35 pmol/L. HoloTC assay for each sample were repeated after polyethylene glycol (PEG) treatment to exclude IgG, IgA and IgM type immune complexes. Also, methylmalonic acid, folate, homocystein, liver, and kidney function tests were obtained. Methylmalonic acid test showed that 42 patients (68.9%) had vitamin B-12 deficiency. Vitamin B-12 levels by HoIoTC assay decreased by 19.2 +/- 11.28% in essential thrombocytosis, 40.0 +/- 9.39% in chronic myeloid leukemia, 30.9 +/- 14.62% in myelofibrosis and 21.2 +/- 11.55% in polycythemia vera patients after PEG treatment. There was significant difference between the averages of groups (p< 0.01). Methylmalonic Acid Test was used as the B-12 status variable. The comparison of ROC curves of HoIoTC before and after PEG showed no statistically significance between area under curves. The optimum cut-off points for both HoloTC before and after PEG were 40.6 pmol/L and 32.1 pmol/L, respectively. Immune complexes may have some effect on HoIoTC assay which has been recently reported to have a superior diagnostic accuracy for vitamin Biz deficiency in patients with MPDs. Although exclusion of immune complexes did not improve its diagnostic performances, effects of exclusion were significantly different between subgroups of MPDs.
dc.language English
dc.publisher AKAD DOKTORLAR YAYINEVI
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Oncology
dc.title Effects of Immune Complexes on Holotranscobalamine Assay of Vitamin B-12 Deficiency in Myeloproliferative Disorders
dc.type Article
dc.identifier.volume 29
dc.identifier.startpage 31
dc.identifier.endpage 37
dc.contributor.department Sakarya Üniversitesi/Tıp Fakültesi/Temel Tıp Bilimleri Bölümü
dc.contributor.saüauthor Cinemre, Fatma Behice
dc.contributor.saüauthor Aydemir, Birsen
dc.contributor.saüauthor Cinemre, Hakan
dc.relation.journal UHOD-ULUSLARARASI HEMATOLOJI-ONKOLOJI DERGISI
dc.identifier.wos WOS:000465618700005
dc.identifier.doi 10.4999/uhod.193047
dc.contributor.author Cinemre, Fatma Behice
dc.contributor.author Aydemir, Birsen
dc.contributor.author Cinemre, Hakan


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