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Investigation of mutations in adeR and adeS gene regions in gentamicine resistant Acinetobacter baumannii isolates

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dc.contributor.authors Atasoy, AR; Ciftci, IH; Petek, M; Terzi, HA;
dc.date.accessioned 2020-02-27T07:19:25Z
dc.date.available 2020-02-27T07:19:25Z
dc.date.issued 2016
dc.identifier.citation Atasoy, AR; Ciftci, IH; Petek, M; Terzi, HA; (2016). Investigation of mutations in adeR and adeS gene regions in gentamicine resistant Acinetobacter baumannii isolates. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 30, 367-360
dc.identifier.issn 1310-2818
dc.identifier.uri https://doi.org/10.1080/13102818.2015.1135082
dc.identifier.uri https://hdl.handle.net/20.500.12619/65435
dc.description.abstract This study evaluated the relationship between aminoglycoside resistance and the active efflux pump and investigated the role of the active efflux pump in resistance mechanisms. In addition, the mutations related to aminoglycoside resistance were investigated in Acinetobacter baumannii isolates obtained from different clinical specimens. The study included 32 A. baumannii isolates. They were identified and their susceptibilities were determined using conventional techniques and an automated system. Total genomic RNA, DNA and cDNA were obtained using commercial extraction kits. Primers for adeR and adeS were designed using sequences in GenBank. All isolates were subjected to polymerase chain reaction (PCR) to determine the presence of adeR and adeS. The PCR products were electrophoresed for the optimization study. Subsequently, real-time PCR was performed to determine the expression levels of the adeR and adeS genes. Sequence analysis of the two adeRS operons in our isolates showed five mutations differing from those of other isolates. Isolate A21 had three mutations: (Tyr31Phe), (Val136Ala) and (Leu142Ile); isolate A24 had two mutations: (Asn115His) and (Leu142Ile). In our study, the examined gene regions that play a role in the resistance mechanisms of A. baumannii were considered important. The results indicated that adeR and adeS expression clearly affect aminoglycoside resistance. However, gene expression alone does not seem sufficient to explain that. These results could help to design improved active efflux pump inhibitors.
dc.language English
dc.publisher TAYLOR & FRANCIS LTD
dc.subject Biotechnology & Applied Microbiology
dc.title Investigation of mutations in adeR and adeS gene regions in gentamicine resistant Acinetobacter baumannii isolates
dc.type Article
dc.identifier.volume 30
dc.identifier.startpage 360
dc.identifier.endpage 367
dc.contributor.department Sakarya Üniversitesi/Tıp Fakültesi/Temel Tıp Bilimleri Bölümü
dc.contributor.saüauthor Atasoy, Ali Rıza
dc.contributor.saüauthor Çiftci, İhsan Hakkı
dc.contributor.saüauthor Terzi, Hüseyin Agah
dc.relation.journal BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
dc.identifier.wos WOS:000372200700022
dc.identifier.doi 10.1080/13102818.2015.1135082
dc.identifier.eissn 1314-3530
dc.contributor.author Atasoy, Ali Rıza
dc.contributor.author Çiftci, İhsan Hakkı
dc.contributor.author Mustafa Petek
dc.contributor.author Terzi, Hüseyin Agah


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