Microbial transformation of androstenedione by Cladosporium sphaerospermum and Ulocladium chartarum

Abstract

In this work, incubations of androstenedione 1 with Cladosporium sphaerospermum MRC 70266 and Ulocladium chartarum MRC 72584 have been reported. C. sphaerospermum MRC 70266 mainly hydroxylated 1 at C-6 beta, accompanied by a hydroxylation at C-15 alpha, a reduction at C-17, a 5 alpha-reduction and oxidations at C-6 and C-16 following hydroxylations. U. chartarum MRC 72584 hydroxylated 1 at C-6 beta, C-7 alpha, C-7 beta and C-14 alpha, accompanied by an oxidation at C-6 following its hydroxylation, a reduction at C-17 and a 5 alpha-reduction. 6 beta,17 beta-Dihydroxyandrost-4-en-3,16-dione 8, one of the metabolites from the incubation of 1 with C. sphaerospermum MRC 70266, was determined as a new compound.