Investigation of inhibitory effects of triazole derivatives on the protease enzymes

Abstract

Triazoles constitute an important class of heterocyclic organic compounds because of their wide range of applications as pharmaceutical agents. They have common mode of action like inhibiting ergosterol synthesis, decreasing adhesion potential of pathogen cells to host tissues and morphogenetic transformation of yeasts to mycelial form. Fungicidal properties of azole agents have been known for many years, however, the investigation of inhibitiory effects of these molecules on various enzymes has increased in recent years. As being the most important group of industrial enzymes, proteases have received significant interest in the food, pharmaceutical and detergent industries. In this study, the inhibition effect of three triazole derivatives on serine (chymotrypsin and trypsin) and cysteine (papain) proteases was examined and IC50 values were determined. Also, the inhibition type for trypsin was established for each molecule separately.