Dye decolorization by Dandelion (Taraxacum officinale) Polyphenol Oxidase immobilized into Alginate Beads

Abstract

In this work, Dandelion (Taraxacum officinale) polyphenol oxidase immobilized on calcium alginate beads was used for the successful and effective decolorization of textile industrial effluent. Polyphenol oxidase (PPO) enzyme was extracted from dandelion (Taraxacum officinale) and immobilized on calcium alginate beads. The immobilized and free enzymes were employed for the decolorization of acid and metal complex dyes. Dye solutions of acid and metal complex dyes were prepared in the concentration 100 mg/L in distilled water and incubated with free and immobilized Dandelion PPO for one hour. The percent decolorization was calculated by taking untreated dye solution. Immobilized PPO was significantly more effective in decolorizing of the dyes as compared to free enzyme. Our results showed that the immobilized Dandelion PPO enzyme could be efficiently used for the removal of synthetic dyes from industrial effluents.