dc.contributor.authors |
Hatipoglu, Huseyin; Erman, Gulay; Toptan, Hande; Koroglu, Mehmet; Altindis, Mustafa |
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dc.date.accessioned |
2022-12-20T13:24:46Z |
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dc.date.available |
2022-12-20T13:24:46Z |
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dc.date.issued |
2022 |
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dc.identifier.issn |
0959-3993 |
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dc.identifier.uri |
http://dx.doi.org/10.1007/s11274-022-03332-2 |
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dc.identifier.uri |
https://hdl.handle.net/20.500.12619/98980 |
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dc.description |
Bu yayının lisans anlaşması koşulları tam metin açık erişimine izin vermemektedir. |
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dc.description.abstract |
In this study, it was aimed to determine the antibiotic susceptibility of bacterial strains by using flow cytometry method by comparing them with current standardized methods. Eleven clinical isolates and 6 standard bacterial strains were included in the study. MIC values were determined by broth microdilution method (BMD), VITEK 2 (R) automated system and flow cytometric method (FCM). FCM was performed with the Accuri C6 flow cytometer. For all strains except P. aeuruginosa ATCC 27853 [BMD-FCM:r = 0.557(p = 0.048); VITEK 2-FCM:r = 0.529(p = 0.063)], E. faecalis ATCC 29212 [BMD-FCM:r = 0.393(p = 0.295); BMD-VITEK 2:r = 0.393(p = 0.295)], and vancomycin-resistant E. faecium clinical isolate [BMD-FCM:r = 0.452(p = 0.063)] r values were in the range of 0.802-0.969 for BMD-FCM (p < 0.001), 0.655-0.941 for BMD-VITEK 2 (p < 0.005) and 0.667-0.953 for FCM-VITEK 2 (p < 0.005). Correlation values of antibiotic susceptibility test results between three methods for Gram-negative bacteria were found as follows; r = 0.927(p < 0.001) for BMD-FCM, r = 0.851(p < 0.001) for BMD-VITEK 2, r = 0.807(p < 0.001) for VITEK 2-FCM. Correlation values were found as follows for Gram positive bacteria; r = 0.848(p < 0.001) for BMD-FCM, r = 0.877(p < 0.001) for BMD-VITEK 2, r = 0.800(p < 0.001) for VITEK 2-FCM. When all bacteria included in the study were evaluated as a total; it was r = 0.911(p < 0.001) for BMD-FCM, r = 0.888(p < 0.001) for BMD-VITEK 2, r = 0.835(p < 0.001) for VITEK 2-FCM. The methicillin resistance of the clinical methicillin resistant S. aureus isolate could not be detected by FCM. It was determined that there was a high level of correlation between methods. FCM shortens the duration of antibiotic susceptibility tests by 12-14 h and gives results within the same day. However, it has not been standardized to be widely used in microbiology laboratories and experienced personnel are needed for its implementation. |
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dc.language |
English |
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dc.language.iso |
eng |
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dc.relation.isversionof |
10.1007/s11274-022-03332-2 |
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dc.subject |
Biotechnology & Applied Microbiology |
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dc.subject |
Antibiotic |
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dc.subject |
Antibiotic resistance |
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dc.subject |
Antibiotic susceptibility |
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dc.subject |
Antibiotic susceptibility test methods |
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dc.subject |
Bacteria |
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dc.title |
Determination of antibiotic susceptibility of bacteria by flow cytometric method |
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dc.contributor.authorID |
Altindis, Mustafa/0000-0003-0411-9669 |
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dc.contributor.authorID |
ERMAN, GULAY/0000-0002-9926-369X |
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dc.identifier.volume |
38 |
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dc.relation.journal |
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY |
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dc.identifier.issue |
9 |
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dc.identifier.doi |
10.1007/s11274-022-03332-2 |
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dc.identifier.eissn |
1573-0972 |
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dc.contributor.author |
Hatipoglu, Huseyin |
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dc.contributor.author |
Erman, Gulay |
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dc.contributor.author |
Toptan, Hande |
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dc.contributor.author |
Koroglu, Mehmet |
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dc.contributor.author |
Altindis, Mustafa |
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dc.relation.publicationcategory |
Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı |
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