dc.date.accessioned |
2021-06-08T09:12:01Z |
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dc.date.available |
2021-06-08T09:12:01Z |
|
dc.date.issued |
2020 |
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dc.identifier.issn |
1759-9660 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12619/96176 |
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dc.description |
Bu yayının lisans anlaşması koşulları tam metin açık erişimine izin vermemektedir. |
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dc.description.abstract |
In the present work, a notably sensitive and selective electrochemical 8-OHdG sensor based on an Ag-TiO2-reduced graphene oxide (rGO) hybrid nanomaterial modified screen-printed electrode (SPE) was developed for 8-hydroxy-2 '-deoxyguanosine (8-OHdG) determination, which is a significant DNA biomarker because it is one of the main products of oxidative DNA damage. The amperometric responses of the fabricated sensor (Ag-TiO2-rGO/SPE) were measured via monitoring the oxidation current of 8-OHdG by using the differential pulse voltammetry (DPV) technique. The effects of the rGO : Ag-TiO2 ratio, amount of Ag-TiO2-rGO on the electrode surface, electrolyte pH, and possible interfering substances on the current response of Ag-TiO2-rGO/SPE were investigated in detail. Under optimized conditions, the current signals linearly correlated with the concentration of 8-OHdG in the range of 0.05-25 mu M (R-2 = 0.9967) with a detection limit of 10 nM. Furthermore, Ag-TiO2-rGO/SPE was successfully tested to determine the 8-OHdG level in a human urine sample (only in a volume of 50 mu L) with satisfactory recovery. |
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dc.language |
English |
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dc.language.iso |
eng |
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dc.publisher |
ROYAL SOC CHEMISTRY |
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dc.relation.isversionof |
10.1039/c9ay02175b |
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dc.rights |
info:eu-repo/semantics/closedAccess |
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dc.subject |
CAPILLARY-ELECTROPHORESIS |
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dc.subject |
PHOTOCATALYTIC ACTIVITY |
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dc.subject |
FACILE SYNTHESIS |
|
dc.subject |
VISIBLE-LIGHT |
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dc.subject |
SENSOR |
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dc.subject |
8-HYDROXYDEOXYGUANOSINE |
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dc.subject |
NANOPARTICLES |
|
dc.subject |
PERFORMANCE |
|
dc.subject |
COMPOSITE |
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dc.subject |
REDUCTION |
|
dc.title |
An Ag-TiO2-reduced graphene oxide hybrid film for electrochemical detection of 8-hydroxy-2 '-deoxyguanosine as an oxidative DNA damage biomarker |
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dc.type |
Article |
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dc.contributor.authorID |
Ozmen, Mustafa/0000-0001-5117-9168 |
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dc.identifier.volume |
12 |
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dc.identifier.startpage |
499 |
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dc.identifier.endpage |
506 |
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dc.relation.journal |
ANALYTICAL METHODS |
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dc.identifier.issue |
4 |
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dc.identifier.doi |
10.1039/c9ay02175b |
|
dc.identifier.eissn |
1759-9679 |
|
dc.contributor.author |
Dhulkefl, Ayad Jirjees |
|
dc.contributor.author |
Atacan, Keziban |
|
dc.contributor.author |
Bas, Salih Zeki |
|
dc.contributor.author |
Ozmen, Mustafa |
|
dc.relation.publicationcategory |
Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı |
|