dc.rights.license |
DOAJ Gold |
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dc.date.accessioned |
2021-06-03T08:20:58Z |
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dc.date.available |
2021-06-03T08:20:58Z |
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dc.date.issued |
2020 |
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dc.identifier.issn |
2147-673X |
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dc.identifier.uri |
www.doi.org/10.4274/mjima.galenos.2020.2020.11 |
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dc.identifier.uri |
https://hdl.handle.net/20.500.12619/95285 |
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dc.description |
Bu yayın 06.11.1981 tarihli ve 17506 sayılı Resmî Gazete’de yayımlanan 2547 sayılı Yükseköğretim Kanunu’nun 4/c, 12/c, 42/c ve 42/d maddelerine dayalı 12/12/2019 tarih, 543 sayılı ve 05 numaralı Üniversite Senato Kararı ile hazırlanan Sakarya Üniversitesi Açık Bilim ve Açık Akademik Arşiv Yönergesi gereğince açık akademik arşiv sistemine açık erişim olarak yüklenmiştir. |
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dc.description.abstract |
Y Introduction: This study aims to assess the in vitro activity of ceftazidime-avibactam (CZA) and ceftolozane-tazobactam (C/T) against the multi-drug resistant (MDR) Klebsiella pneumoniae. Furthermore, we aimed to determine the types of carbapenemase enzyme responsible for carbapenem resistance and compare the activity of ceftolozane-tazobactam and CZA according to the types of carbapenemase enzymes produced. Materials and Methods: Twenty-two MDR isolates were investigated in the study. The identification and antimicrobial susceptibilities of the isolates were performed by VITEK 2 (BioMerieux, France) automated system. The activity of C/T and CZA was determined by the gradient strip test (Liofilchem MIC strip test, Italy). In all K. pneumoniae isolates, bla(IMP-1), bla(KPC), bla(NDM-1), bla(OXA-48) and bla(VIM) gene regions encoding the carbapenemase enzyme were investigated by using Xpert CARBA-R test kits (Cepheid, Sunnyvale, CA, USA) of the Gene-Xpert (R) system. Results: Ceftolozane-tazobactam resistance was detected in 21 (95%) isolates, whereas CZA resistance was detected in six (27%) isolates. Among the 22 K. pneumoniae isolates, NDM-1 gene region was detected in three, NDM-1+OXA-48 gene region was detected in two, OXA-48 was detected in eight, and KPC gene region was detected in nine isolates. VIM and IMP-1 gene regions were not detected. Ceftolozane-tazobactam resistance was detected in seven isolates, whereas CZA resistance was not detected among the eight K. pneumoniae isolates producing only OXA-48. NDM-1 and OXA-48 co-producing isolates were detected resistant to C/T and CZA with high minimum inhibitory concentration (MIC) levels (MIC >= 256 mu g/ml). All NDM-1 producing isolates had high MIC levels (MIC >= 256 mu g/ml) to both C/T and CZA. Ceftazidime-avibactam resistance was detected in only one isolate, whereas C/T resistance was detected in all K. pneumoniae isolates producing KPC. Ceftolozane-tazobactam was detected inefficient whereas CZA was found very efficient in MDR K. pneumoniae isolates producing KPC. Conclusion: According to the obtained data, we detected the in vitro antibacterial activity of CZA against the MDR K. pneumoniae isolates was superior to that of C/T. Ceftolozane-tazobactam was found to be weakly efficient, whereas CZA was found to be highly efficient against the MDR K. pneumoniae isolates producing OXA-48 and KPC. Because avibactam can inhibit the activity of KPC-type carbapenemase, the combination drug CZA should be considered to be effective in the treatment of KPC-type carbapenemase-producing strains but noneffective against NDM-type carbapenemase-producing strains. |
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dc.language |
English |
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dc.language.iso |
İngilizce |
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dc.publisher |
GALENOS YAYINCILIK |
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dc.relation.isversionof |
10.4274/mjima.galenos.2020.2020.11 |
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dc.rights |
info:eu-repo/semantics/openAccess |
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dc.subject |
PSEUDOMONAS-AERUGINOSA |
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dc.subject |
ANTIMICROBIAL ACTIVITY |
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dc.subject |
ENTEROBACTERIACEAE |
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dc.subject |
SURVEILLANCE |
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dc.subject |
INFECTIONS |
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dc.subject |
HOSPITALS |
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dc.subject |
AGENTS |
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dc.subject |
Klebsiella pneumoniae |
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dc.subject |
multi-drug resistant |
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dc.subject |
ceftazidime-avibactam |
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dc.subject |
ceftolozane-tazobactam |
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dc.subject |
carbapenemase enzymes |
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dc.title |
Evaluation of In Vitro Activity of Ceftolozane-tazobactam and Ceftazidime-avibactam Against Carbapenemase-producing Multi-drug Resistant Klebsiella pneumoniae Isolates |
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dc.type |
Article |
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dc.contributor.authorID |
Altindis, Mustafa/0000-0003-0411-9669 |
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dc.identifier.volume |
9 |
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dc.relation.journal |
MEDITERRANEAN JOURNAL OF INFECTION MICROBES AND ANTIMICROBIALS |
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dc.identifier.wos |
WOS:000617445000003 |
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dc.identifier.doi |
10.4274/mjima.galenos.2020.2020.11 |
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dc.contributor.author |
Terzi, Huseyin Agah |
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dc.contributor.author |
Aydemir, Ozlem |
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dc.contributor.author |
Demiray, Tayfur |
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dc.contributor.author |
Koroglu, Mehmet |
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dc.contributor.author |
Altindis, Mustafa |
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dc.relation.publicationcategory |
Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı |
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